Synthesis and characterization of hydroxyapatite modified with (9r)-9-hydroxystearic acid

Mamigonian Bessa, Luiza (2014) Synthesis and characterization of hydroxyapatite modified with (9r)-9-hydroxystearic acid. [Laurea magistrale], Università di Bologna, Corso di Studio in Advanced spectroscopy in chemistry [LM-DM270]
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Abstract

(9R)-9-hydroxystearic acid (9R-HSA) has been proven to have antitumoral activity because it is shown to inhibit histone deacetylase 1, an enzyme which activates DNA replication, and the (R)-enantiomer has been shown to be more active than the (S)-enantiomer both in vitro and by molecular docking. Hydroxyapatite is the main mineral component of bone and teeth and has been used for over 20 years in prostheses and their coating because it is biocompatible and bioactive. The goal of incorporating 9R-HSA into hydroxyapatite is to have a material that combines the bioactivity of HA with the antitumoral properties of 9R-HSA. In this work, 9R-HSA and its potassium salt were synthesized and the latter was also incorporated into hydroxyapatite. The content of (R)-9-hydroxystearate ion incorporated into the apatitic structure was shown to be a function of its concentration in solution and can reach values higher than 8.5%. (9R)-9-hydroxystearic acid modified hydroxyapatite was extensively characterized to determine the effect of the incorporation of the organic molecule. This incorporation does not significantly alter the unit cell but reduces the size of both the crystals as well as the coherent domains, mainly along the a-axis of hydroxyapatite. This is believed to be due to the coordination of the negatively charged carboxylate group to the calcium ions which are more exposed on the (100) face of the crystal, therefore limiting the growth mainly in this direction. Further analyses showed that the material becomes hydrophobic and more negatively charged with the addition of 9R-HSA but both of these properties reach a plateau at less than 5% wt of 9R-HSA.

Abstract
Tipologia del documento
Tesi di laurea (Laurea magistrale)
Autore della tesi
Mamigonian Bessa, Luiza
Relatore della tesi
Scuola
Corso di studio
Parole chiave
hydroxyapatite 9-hydroxystearic acid nuclear magnetic resonance spectroscopy X-ray powder diffraction
Data di discussione della Tesi
25 Luglio 2014
URI

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