Evaluation of microplastic content in faecal sample from hospitalized loggerhead sea turtles (Caretta caretta) of the North Adriatic Sea.

Microplastics represent persistent and highly dispersal pollutants in the marine environment. The knowledge about ingestion and relative effects in large marine vertebrates, such as sea turtles, is still fragmentary. To date, studies on MP accumulation in these animals were based on the analysis of gastrointestinal tracts obtained after necropsy. In this study, we developed a protocol that allows the study of the MP through faecal material from alive turtles, that can be used in rescue centres in several locations. Specifically, faecal samples from 45 specimens of Caretta caretta, rescued along the coast of Emilia-Romagna and Marche (Italy) from 2016 to 2019 were assessed for MP content through different hospitalization periods. A unique sample was collected from 24 animals, while for 21 specimens two samples were obtained. This allowed us to quantify and characterize the MPs and to evaluate putative differences in the excreted particles during the hospitalization period. All individuals showed MPs in the faeces, for an average value of 6 ± 6.09 particles/animal. The maximum number of particles found in a single individual is 34 MPs. Filamentous particles were the most represented shape and transparent/white and red colours prevailed over the other colours. Analyses of data about first sampling show a significant negative correlation between MP number and turtle’s size. This could be due to the different feeding behaviours that occur in these animals according to their life stage. The absence of significant differences between classes and categories of shape and colours particles could be due to the high contamination of the preys in the Adriatic Sea. The absence of significant differences between the first and second sampling (where available), suggests that during the hospitalization period there might have been external influences that affected the results, including MP intake with diet, which would be considered in future studies.