Testing viability of cells grown in vitro onboard customized electrospun membranes intended for tissue engineering applications

This study explores the potential of customized electrospun membranes to support cell growth for tissue engineering applications, a promising solution to manufacture replacement tissues from a patient's own cells, minimizing rejection and achieving better organ compatibility. In particular, it evaluates the interaction of MC3T3 cells with electrospun membranes made of a novel synthetic polymer. Initially, an indirect cytotoxicity assay assessed the biocompatibility of the electrospun membranes with MC3T3 cells grown in cell culture grade plasticware. Further, cells were seeded and cultured onto these membranes, which were supported by 3D-printed holder timbrels. Cell adhesion and proliferation was subsequently evaluated using a PrestoBlue®-based assay at various time points. A daily standard curve ensured accurate quantification of cell proliferation. Results highlight that electrospun membranes support cell adhesion and proliferation, although their adhesion and rate of proliferation is slower when compared to a traditional plastic substrate.