Monoclonal antibodies purification via Protein G and protein A affinity chromatography

realization of a pilot scale affinity protein G chromatography for the purification of small volumes of supernatant from different harvests from the same antibody clone. The objective was to make a study on the productivity of each harvest, investigating for a dilution effect in case of an increasing number of harvests, and to have a rapid retention kit for the quality control of the purified antibody. Moreover, an identical column packed with protein A has been tested with the same products, in order to see if there was space for a yield improvement, and a further scale-up.