Development of urease enzyme inhibitors and assessment of their efficacy and ecotoxicity

Marullo, Jacopo (2020) Development of urease enzyme inhibitors and assessment of their efficacy and ecotoxicity. [Laurea magistrale], Università di Bologna, Corso di Studio in Analisi e gestione dell'ambiente [LM-DM270] - Ravenna
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Abstract

This work study proposes novel and natural inhibitors of the enzyme urease, as more sustainable alternatives to the synthetic ones. Specifically, Deep Eutectic Solvents (DES) were used as an extractants and carriers of polyphenols extracted from waste biomass enriched in antioxidant compounds. The polyphenolic extracts with DES have been tested on lab-scale experiments to verify their effect on the reduction of the hydrolysis rate of urea-based fertilizers catalyzed by urease. The phytotoxicity and the soil ecotoxicity of DES and polyphenols formulations were then tested. DES resulted promising in terms of polyphenols extraction ability from biomass and as carriers of bioactive compounds in the agricultural field, showing non-damaging effects on plants (Avena sativa) and microarthropods in soil.

Abstract
Tipologia del documento
Tesi di laurea (Laurea magistrale)
Autore della tesi
Marullo, Jacopo
Relatore della tesi
Correlatore della tesi
Scuola
Corso di studio
Ordinamento Cds
DM270
Parole chiave
urease, deep eutectic solvents, polyphenols, waste biomass, phytotoxicity, soil ecotoxicity, green chemistry
Data di discussione della Tesi
10 Dicembre 2020
URI

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